You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1101195 |
---|---|
Category | Proteins |
Description | Interleukin 3 is also known as IL-3, is a protein that in humans is encoded by the IL3 gene. Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. The IL-3 receptor is a heterodimer of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. [3] Binding of IL-3 can also involve substitution of the βc by a βIL-3-chain that appears to be specific for IL-3 . Binding of IL-3 to its cognate receptor(s) induces activation of Jak2, phosphorylation of multiple Stats, and the PI3K/Akt pathway. IL-3 may play an important role in the development of airway inflammation associated with asthma. The human IL-3 gene encodes a protein 152 amino acids long, and the naturally occurring IL-3 is glycosylated. The human IL-3 gene is located on chromosome 5, only 9 kilobases from the GM-CSF gene, and its function is quite similar to GM-CSF. |
Reactivity | Mouse |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 17.6 kDa |
Target | IL-3 |
Source | Mouse IL-3 Protein, His Tag (orb1101195) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Cys 166 (Accession # P01586-1). |
Expression System | HEK293 |
Biological Activity | Immobilized Mouse IL-3 R alpha, His Tag on CM5 Chip can bind Mouse IL-3 Protein, His Tag with an affinity constant of 0.929 μM as determined in a SPR assay (Biacore T200) (QC tested). |
Expression Region | Ala 27 - Cys 166 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_034686.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL3,MCGF,MGC79398,MGC79399,MULTI-CSF,Interleukin-3 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Mouse IL-3 R alpha, His Tag on CM5 Chip can bind Mouse IL-3 Protein, His Tag (Cat. No. orb1101195) with an affinity constant of 0.929 μM as determined in a SPR assay (Biacore T200) (QC tested).
> 98% as determined by SDS-PAGE and HPLC. | |
14.8 kDa | |
E.Coli |
Unconjugated | |
95% | |
19.5 kDa | |
Human IL-10, His Tag (orb334903) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # NP_000563.1). |
Unconjugated | |
95% | |
18.6 kDa | |
Human IL-10, premium grade (orb1496180) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # P22301-1). |
Unconjugated | |
90% | |
20.6 kDa | |
Cynomolgus IL-10 Protein, His Tag (orb1496244) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # P79338). |
Unconjugated | |
92% | |
19.6 kDa | |
Mouse IL-10, His Tag (orb334904) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Ser 178 (Accession # NP_034678). |
Filter by Rating