Mouse IL-18 antibody

• Catalog Number: orb345221
• Category: Antibodies
• Description: Mouse IL-18 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with native 157 aa mouse IL-18 produced in E.coli.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:1,000 - 1:5,000, IHC: User Optimized, IF: 1:50-1:200, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This is an IgG preparation of whole rabbit serum purified by protein A chromatography using a low endotoxin methodology. This antibody is primarily directed against mature 18,000 MW mouse IL-18 and is useful in determining its presence in various assays. This antibody will also recognize the 24,000 inactive precursor form of mouse IL-18. In general, this antibody also detects rat IL-18 in the same formats using similar dilutions. A control of similarly diluted LOW ENDOTOXIN CONTROL RABBIT IgG (code # 011-001-297) is recommended.
• Conjugation: Unconjugated
• UniProt ID: P70380
• NCBI: P70380.2
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: None
• Alternative names: rabbit anti-IL-18 antibody, rabbit anti-interleuki
• Note: For research use only
• Application notes: Anti-Mouse IL-18 has been tested in immunohistochemistry and immunofluorescence and is suitable for use in neutralizations, ELISA, and immunoblotting. Although untested, this reagent may be useful for radioimmunoassays, flow cytometry and immunoprecipitation. It recognizes the 18,000 MW mature (active) IL-18. Reactivity in other immunoassays is unknown.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of mouse colon sections using Mouse IL-18 antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using Mouse IL-18 antibody