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Catalog Number | orb345220 |
---|---|
Category | Antibodies |
Description | Mouse IL-18 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with native 157 aa mouse IL-18 produced in E.coli. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:1,000 - 1:5,000, IHC: User Optimized, IF: 1:50-1:200, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This is an IgG preparation of whole rabbit serum purified by protein A chromatography using a low endotoxin methodology. This antibody is primarily directed against mature 18,000 MW mouse IL-18 and is useful in determining its presence in various assays. This antibody will also recognize the 24,000 inactive precursor form of mouse IL-18. In general, this antibody also detects rat IL-18 in the same formats using similar dilutions. A control of similarly diluted LOW ENDOTOXIN CONTROL RABBIT IgG (code # 011-001-297) is recommended. |
Conjugation | Unconjugated |
UniProt ID | P70380 |
NCBI | P70380.2 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | None |
Alternative names | rabbit anti-IL-18 antibody, rabbit anti-interleuki Read more... |
Note | For research use only |
Application notes | Anti-Mouse IL-18 has been tested in immunohistochemistry and immunofluorescence and is suitable for use in neutralizations, ELISA, and immunoblotting. Although untested, this reagent may be useful for radioimmunoassays, flow cytometry and immunoprecipitation. It recognizes the 18,000 MW mature (active) IL-18. Reactivity in other immunoassays is unknown. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of mouse colon sections using Mouse IL-18 antibody
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using Mouse IL-18 antibody
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