Mouse IgM (mu chain) Antibody

• Catalog Number: orb347519
• Category: Antibodies
• Description: Mouse IgM (mu chain) antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 2.2 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgM and Mouse Serum. No reaction was observed against other mouse heavy or light chain proteins.
• Immunogen: Mouse IgM whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Application notes: Anti-Mouse IgM mu heavy chain antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: Rabbit Anti-Mouse IgM (mu chain) Antibody, Rabbit
• Note: For research use only

ELISA Results of Rabbit Anti-Mouse IgM Antibody tested against purified Mouse IgM. Each well was coated in duplicate with 10 µg of Mouse IgM (p/n orb346283). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Rabbit IgG mx10 HRP conjugated (p/n orb347654), and TMB substrate (p/n orb348651).