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Catalog Number | orb1496229 |
---|---|
Category | Antibodies |
Description | Mouse IgG2a Kappa Isotype Control |
Species/Host | Human |
Clonality | Recombinant |
Reactivity | Mouse |
Isotype | Mouse IgG2a | Mouse kappa |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
Target | IgG control |
Source | Mouse IgG2a Kappa Isotype Control (mAb) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with Mouse IgG2a constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with DNP. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Each laboratory should determine an optimum working titer for use in its particular application. |
Expiration Date | 12 months from date of receipt. |
ELISA, FC, FLISA, IF, SDS-PAGE, WB | |
Unconjugated | |
Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE. | |
Mouse |
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