Mouse IgG2a Kappa isotype Control Antibody

SKU: orb343763

Description

Mouse IgG2a Kappa isotype Control Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA, FC, SDS-PAGE, WB
• Application Notes:
  Mouse IgG2a kappa isotype control has been tested in SDS-Page, ELISA, and western blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

• Source: Mouse
• Biological Origin: Mouse
• Isotype: IgG2a
• Purity: Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.009 mg/mL
• Disclaimer: For research use only

Alternative Names

Mouse IgG2a isotype control, Mouse IgG2a subclass isotype control, Mouse IgG2a Kappa

SDS-PAGE of Mouse IgG2a Kappa Isotype Control. Lane 1: Mouse IgG2a Kappa Isotype Control, Non-reduced. M: Opal Pre-stained Ladder. Lane 2: Mouse IgG2a Kappa Isotype Control, Reduced. Load: 1.0 µg per lane. Predicted/Observed: 140 kDa Non-reduced, 55 and 25 Reduced.