Mouse IgG2a (Gamma 2a chain) Antibody Texas Red Conjugated

SKU: orb347613

Description

Mouse IgG2a (Gamma 2a chain) antibody (Texas Red)

Images & Validation

• Tested Applications: FC, FLISA, IF, WB
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Mouse
• Application Notes:
  Anti-Mouse IgG2a Texas Red Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG2a heavy chain
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
• Conjugation: Texas Red

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Mouse IgG2a (Gamma 2a chain) Antibody Texas Red Conjugated, Rabbit Anti Mouse IgG2a Texas Red Conjugated Antibody

Isolated hADSCs (IADSCs) were differentiated into SMCs using retinoic acid (RA), heparin was used as a positive control, while SK-UT-1 cells was used as a SMC control. Expression of SMC markers smooth muscle alpha actin (SM-αa, red, Texas Red Conjugated anti-Mouse IgG2, γ2a chain specific), desmin (green, Fluorescein Conjugated anti-Mouse IgG1, γ1 chain specific), smooth muscle myosin heavy chain (SM-MHC, red, Texas Red Conjugated anti-Mouse κ, kappa chain specific), and smoothelin (green, Fluorescein Conjugated anti-Mouse IgG1, γ1 chain specific) in differentiated SMCs was determined by indirect immunofluorescence. Nuclei were counter stained with DAPI (blue). Expression of all four markers can be seen in all the cells, particularly in RA differentiated SMCs.

Western blot of Texas Red™ conjugated Rabbit Anti-Mouse IgG2a (Gamma 2a chain) secondary antibody. Lane 1: Mouse IgG2a. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red™ rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2a (Gamma 2a chain). Other band(s): None.