Mouse IgG2a (Gamma 2a chain) Antibody Fluorescein Conjugated

SKU: orb347541

Description

Mouse IgG2a (Gamma 2a chain) antibody (FITC)

Images & Validation

• Tested Applications: DOT, FC, FLISA, IF, WB
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Mouse
• Application Notes:
  Anti-Mouse IgG2a Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG2a heavy chain
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) against a panel of immobilized mouse and human serum proteins resulting in a conjugated antibody designed for flow cytometry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Mouse IgG2a (gamma 2a chain) Antibody fluorescein Conjugated, Rabbit Anti-Mouse IgG2a Antibody FITC Conjugation

Dot Blot results of Rabbit Anti-Mouse IgG2a Antibody Fluorescein Conjugated. Dots are Mouse IgG2a at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Rabbit Anti-Mouse IgG2a FITC at 1 µg/ml for 1hr at RT. Secondary Antibody: none.

Western blot of Fluorescein conjugated Rabbit Anti-Mouse IgG2a (Gamma 2a chain) secondary antibody. Lane 1: Mouse IgG2a. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2a (Gamma 2a chain). Other band(s): None.