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Catalog Number | orb347526 |
---|---|
Category | Antibodies |
Description | Mouse IgG1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, IHC, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | Mouse IgG1 heavy chain |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum, Mouse IgG and Mouse IgG1. Specificity was confirmed by ELISA. For IgG2a, IgG2b and IgG3 reactivity was less than 5% against target (IgG1, IgG). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody, R Read more... |
Note | For research use only |
Application notes | Mouse IgG1 secondary antibody is available in a variety of formats. Anti-Mouse IgG1 Antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays. |
Expiration Date | 12 months from date of receipt. |
ICC, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, Enzyme Assay, ICC, IHC, IP, WB | |
Bovine, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat | |
Rat | |
Monoclonal | |
Unconjugated |
ELISA, Enzyme Assay, ICC, IHC, IP, WB | |
Bovine, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat | |
Rat | |
Monoclonal | |
Unconjugated |
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