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Catalog Number | orb347427 |
---|---|
Category | Antibodies |
Description | Mouse IgG (H&L) antibody (Peroxidase) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat. |
Concentration | 2.0 mg/mL |
Dilution range | ELISA: 1:50,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | MOUSE IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. |
Conjugation | HRP |
Storage | Store HRP Conjugated Mouse Secondary Antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | Goat Anti-Mouse IgG Secondary Antibody Peroxidase Read more... |
Note | For research use only |
Application notes | Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse HRP antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
ELISA, ICC, IHC, WB | |
Mouse | |
Goat | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Mouse | |
Goat | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Mouse | |
Rabbit | |
Polyclonal | |
HRP |
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