Mouse IgG (H&L) antibody (Peroxidase)

• Catalog Number: orb347544
• Category: Antibodies
• Description: Mouse IgG (H&L) antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:50,000 - 1:300,000, IHC: 1:500 - 1:2,500, WB: 1:10,000 - 1:30,000
• Form/Appearance: Lyophilized
• Purity: HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Conjugation: HRP
• Storage: Store mouse secondary antibody conjugate at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Rabbit Anti-Mouse IgG Secondary Antibody Peroxidas
• Note: For research use only
• Application notes: Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in rabbit detects specifically Mouse IgG whole molecule. Anti-Mouse IgG peroxidase antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of separated on a 4-20% gradient gel and then transferred to Whatman Protran BA 85 nitrocellulose (0.45 um pore size) using Mouse IgG (H&L) antibody (Peroxidase)