Mouse IgG Antibody

• Catalog Number: orb346279
• Category: Proteins
• Description: Mouse IgG Antibody
• Tested applications: ELISA, FC, IHC, IP, SDS-PAGE, WB
• Isotype: IgG
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IP: User Optimized, WB: User Optimized
• Form/Appearance: Lyophilized
• Purity: Mouse IgG was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.
• Conjugation: Unconjugated
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store purified Mouse IgG at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Immunoglobulin G from Mouse, IgG from Mouse, purif
• Note: For research use only
• Application notes: Mouse IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 1: 5 µL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 3: Reduced Mouse F(c) Fragment (p/n orb346280). Lane 4: Reduced Mouse F(ab) Fragment (p/n orb346282). Lane 5: Mouse IgM Kappa Myeloma Protein (p/n orb346283). Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

SDS-Page of Mouse IgG whole molecule. Lane 1: Mouse IgG reduced. Lane 2: Molecular Weight Marker. Lane 3: Mouse IgG non-reduced. Load: 1 µg per lane. Predicted/Observed size (non-reduced): 160 kDa, 160 kDa. Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa.