Mouse IgG Antibody

SKU: orb346279

Description

Mouse IgG Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Mouse IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Key Properties

• Source: Mouse
• Biological Origin: Mouse
• Isotype: IgG
• Purity: Mouse IgG was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store purified Mouse IgG at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 10.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Immunoglobulin G from Mouse, IgG from Mouse, purified polyclonal IgG antibody

SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). MW: 5 µl Opal Prestained Marker. Lane 1: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 2: Reduced Mouse F(c) Fragment (p/n orb346280). Lane 3: Reduced Mouse F(ab) Fragment (p/n orb346282). Lane 4: Mouse IgM Kappa Myeloma Protein. Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

SDS-Page of Mouse IgG whole molecule. Lane 1: Mouse IgG reduced. Lane 2: Molecular Weight Marker. Lane 3: Mouse IgG non-reduced. Load: 1 µg per lane. Predicted/Observed size (non-reduced): 160 kDa, 160 kDa. Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa.