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Description
Research Area
Images & Validation
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| Application Notes |
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Key Properties
−| Source | Escherichia Coli |
|---|---|
| Biological Activity | The ED50, calculated by the dose-dependent proliferation of mouse AML5 cells is 4.96ng/ml, corresponding to a specific activity of 2.0x105 units/mg. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Protein Sequence | MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ |
Storage & Handling
−| Storage | Stability: Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt-3 Ligand should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
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| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | The FLT3L Mouse protein was lyophilized from sterile filtered solution containing 10mM sodium phosphate, pH 7.5. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Human FLT3 Ligand Protein, mFc-His Tag [orb689394]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 45.6 kDa after removal of the signal peptide.
Mammalian
50 μg, 10 μg, 100 μgFlt3 ligand/FLT3LG Mouse Monoclonal Antibody [orb2974683]
ELISA, FC
Human
Mouse
Monoclonal
Unconjugated
50 μg, 100 μgRecombinant Mouse Flt3 ligand/FLT3L Protein, N-His [orb2964273]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
20.81 kDa
1 mg, 50 μg, 100 μgRecombinant Mouse FLT3LG Protein [orb3002222]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 45.5 KDa. Observed: 51-66 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Mouse FLT3LG Protein [orb3002399]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)
Predicted: 19.4 KDa. Observed: 22-30 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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