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Mouse FGF basic protein
Catalog Number: orb80024
| Catalog Number | orb80024 |
|---|---|
| Category | Proteins |
| Description | Mouse FGF basic protein |
| Tested applications | FA, HPLC, SDS-PAGE |
| Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Conjugation | Unconjugated |
| Target | Mouse FGF basic |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Fibroblast Growth Factor in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | The sequence of the first six N-terminal amino acids was determined and was found to be Met-Pro-Ala-Leu-Pro-Glu. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 range= 0.1ng/ml, determined by the dose-dependant proliferation of BALB/3T3 cells. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | FGF-b was lyophilized from 5mM NaP, pH7.5 and 50mM NaCl. |
| Alternative names | HBGH-2 protein, HBGF-2 protein, Prostatropin prote Read more... |
| Note | For research use only |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.885 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of FGF2 as Reference Standard. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse FGF basic protein
- Item 1 of 1
Mouse Fgf-2 protein [orb704601]
Greater than 90% as determined by SDS-PAGE.
20.3 kDa
E.coli
20 μg, 100 μg, 1 mg - Item 1 of 1
Mouse Fgf2 protein [orb594760]
Greater than 95% as determined by SDS-PAGE.
17.15 kDa
E.coli
500 μg, 1 mg, 10 μg, 50 μg 
Mouse FGF2 protein [orb391730]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
E. coli
500 μg, 50 μg, 10 μgMouse Fgf2 Protein [orb1476888]
Greater than 85% as determined by SDS-PAGE.
18.9 kDa
Yeast
1 mg, 20 μg, 100 μg
