Mouse DHFR Protein

• Catalog Number: orb425110
• Category: Proteins
• Description: Recombinant of mouse DHFR protein
• Form/Appearance: Sterile Filtered colorless solution.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Protein Sequence: MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD
• Source: Escherichia Coli
• Biological Activity: Specific activity is > 0.2 units/mg, in which one unit will convert 1.0 umole of 7,8 –dihydrofloate and beta-NADPH to 5,6,7,8-tetrahydrofloate and beta-NADP per min at pH 6.5 at 25C.
• Storage: Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles
• Buffer/Preservatives: The DHFR solution (1 mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.
• Alternative names: Dihydrofolate reductase, DHFR, DHFRP1, AA607882, A
• Note: For research use only
• Application notes: Enzymes
• Expiration Date: 6 months from date of receipt.