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Catalog Number | orb418700 |
---|---|
Category | Proteins |
Description | Recombinant Mouse Chymotrypsin-like elastase family member 3B |
Tag | N-terminal 6xHis-tagged and C-terminal Myc-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Purity | Greater than 85% as determined by SDS-PAGE. |
MW | 29.6 kDa |
UniProt ID | Q9CQ52 |
Protein Sequence | VVNGEEAVPHSWPWQVSLQYEKDGSFHHTCGGSLITPDWVLTAGHCISTSRTYQVVLGEHERGVEEGQEQVIPINAGDLFVHPKWNSMCVSCGNDIALVKLSRSAQLGDAVQLACLPPAGEILPNGAPCYISGWGRLSTNGPLPDKLQQALLPVVDYEHCSRWNWWGLSVKTTMVCAGGDIQSGCNGDSGGPLNCPADNGTWQVHGVTSFVSSLGCNTLRKPTVFTRVSAFIDWIEETIANN |
Protein Length | Full Length of Mature Protein |
Source | Yeast |
Biological Origin | Mus musculus (Mouse) |
Expression Region | 28-269aa |
Endotoxins | Not test. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Alternative names | Elastase IIIB Elastase-3B Protease E Ela3, Ela3b Read more... |
Note | For research use only |
Application notes | Tag Info: N-terminal 6xHis-tagged and C-terminal Myc-taggedExpression Region: 28-269aaSequence Info: Full Length |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Cela3b.
Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Cela3b.
Greater than 90% as determined by SDS-PAGE. | |
31.1 kDa | |
E.coli |
98.00% | |
29.6 kDa (predicted) |
98.00% | |
31.1 kDa (predicted) |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
26.5 kDa | |
E.Coli |