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Catalog Number | orb391398 |
---|---|
Category | Proteins |
Description | Recombinant mouse CD28 protein is produced by our mammalian expression system and the target gene encoding Asn20-Lys149 is expressed with a Fc, 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CD28 |
Entrez | 12487 |
UniProt ID | P31041 |
Protein Sequence | NKILVKQSPL LVVDSNEVSL SCRYSYNLLA KEFRASLYKG VNSDVEVCVG NGNFTYQPQF RSNAEFNCDG DFDNETVTFR LWNLHVNHTD IYFCKIEFMY PPPYLDNERS NGTIIHIKEK HLCHTQSSPK VDDIEGRMDE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | T-cell-specific surface glycoprotein CD28; TP44; C Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
17.0 kDa | |
Mouse PD-1, His Tag (orb257763) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_032824). |
Unconjugated | |
95% | |
51.4 kDa | |
Mouse PD-L1, Fc Tag (orb257764) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693). |
Unconjugated | |
95% | |
25.6 kDa | |
Mouse B7-1, His Tag (orb383532) is expressed from human 293 cells (HEK293). It contains AA Val 38 - Lys 245 (Accession # Q00609-1). |
Unconjugated | |
95% | |
50.3 kDa | |
Mouse B7-1, Fc Tag (orb257961) is expressed from human 293 cells (HEK293). It contains AA Val 38 - Lys 245 (Accession # Q00609-1). |
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