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Catalog Number | orb424265 |
---|---|
Category | Proteins |
Description | Recombinant of mouse BCA-1 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | The BCA1 protein was lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA. |
Purity | Greater than 97.0% as determined by SDS-PAGE. |
Protein Sequence | ILEAHYTNLK CRCSGVISTV VGLNIIDRIQ VTPPGNGCPK TEVVIWTKMK KVICVNPRAK WLQRLLRHVQ SKSLSSTPQA PVSKRRAA |
Application notes | Chemokines |
Source | Escherichia Coli |
Biological Activity | The ED50, as measured by its ability to chemoattract human CXCR5-transfected mouse BaF3 cells, is less than 2µg/ml. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized CXCL13 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized BCA1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BCA1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Alternative names | C-X-C motif chemokine 13, B lymphocyte chemoattrac Read more... |
Note | For research use only |
> 97% by SDS-PAGE and HPLC analyses. | |
10.3 kDa, a single non-glycosylated polypeptide chain containing 87 amino acids. | |
Escherichia coli. |
> 95% | |
11.7 kDa (predicted). Due to glycosylation, the protein migrates to 16-20 kDa based on Tris-Bis PAGE result. |
98.00% | |
37.1 kDa (predicted). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |