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Mouse anti Human CD4 FITC - CD27 PE
Catalog Number: orb198166
| Catalog Number | orb198166 |
|---|---|
| Category | Antibodies |
| Description | Mouse polyclonal antibody to CD4, CD27 (FITC, PE) |
| Species/Host | Mouse |
| Isotype | IgG1 (F)/IgG1 (PE) |
| Conjugation | FITC/RPE |
| Reactivity | Human |
| Form/Appearance | Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein |
| Concentration | Titered for flow cytometry |
| Buffer/Preservatives | Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein |
| Immunogen | CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes. |
| UniProt ID | P26842, P01730 |
| Tested applications | FACS |
| Application notes | PBMC: Add10 µl of MAB/106^ PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
| Antibody Type | Primary Antibody |
| Storage | Product should be stored at 4-8°C. DO NOT FREEZE |
| Alternative names | CD4 FITC - CD27 PE |
| Hazard Information | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. |
| Note | For research use only |
