Mono-methyl-HIST1H1C (K186) antibody

• Catalog Number: orb416622
• Category: Antibodies
• Description: Mono-methyl-HIST1H1C (K186) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IF, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Peptide sequence around site of Mono-methyl-Lys (186) derived from Human Histone H1.2
• Dilution range: WB:1:500-5000, IF:1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HIST1H1C
• UniProt ID: P16403
• Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Alternative names: Anti-Histone H1.2 antibody, anti- Histone H1c anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Mono-methyl-HIST1H1C (K186) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using

Western blot aanalysis of MCF-7 whole cell lysate, Jurkat whole cell lysate, HEK293 whole cell lysateusing Mono-methyl-HIST1H1C (K186) antibody

Immunofluorescence staining of MCF-7 cells using Mono-methyl-HIST1H1C (K186) antibody