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HIST1H1C Antibody
Description
Images & Validation
−| Tested Applications | ChIP, ELISA, IF, WB |
|---|---|
| Dilution range | WB:1:500-5000, IF:1:50-200 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Peptide sequence around site of Mono-methyl-Lys (186) derived from Human Histone H1.2 |
| Target | HIST1H1C |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol |
| Disclaimer | For research use only |
Alternative Names
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Mono-methyl-HIST1H1C (K186) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using

Western blot aanalysis of MCF-7 whole cell lysate, Jurkat whole cell lysate, HEK293 whole cell lysateusing Mono-methyl-HIST1H1C (K186) antibody

Immunofluorescence staining of MCF-7 cells using Mono-methyl-HIST1H1C (K186) antibody
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UniProt Details
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Protocol Information
HIST1H1C Antibody (orb416622)
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