Monkey IgG (gamma chain) Antibody Rhodamine Conjugated

SKU: orb348007

Description

Monkey IgG (gamma chain) antibody (TRITC)

Images & Validation

• Tested Applications: DOT, FC, FLISA, IF, WB
• Dilution Range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Monkey
• Application Notes:
  Anti-Monkey IgG Rhodamine Conjugated Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Monkey IgG gamma heavy chain
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgG and Monkey Serum.
• Conjugation: TRITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Monkey IgG (gamma chain) rhodamine conjugated antibody, goat anti-Monkey IgG gamma antibody TRITC conjugation

This table displays additional reactivity among various species of serum and immunoglobulin. A (+) indicates antibody reactivity to the corresponding target.

Western Blot showing detection of Monkey IgG Gamma Chain. 100 ng of Monkey IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 5% Blotto (p/n orb348624) 30 min at 20°C, Anti-Monkey IgG (gamma chain) (GOAT) Antibody Rhodamine Conjugated (p/n orb348007) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n orb348637). Molecular weight markers are in lane 1.