MOG Rabbit Polyclonal Antibody

• Catalog Number: orb11068
• Category: Antibodies
• Description: MOG Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Guinea pig, Human, Porcine
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse MOG (35-55/247aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 24 kDa
• Target: MOG
• UniProt ID: Q61885
• RRID: AB_10747117
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: MOG_HUMAN; Myelin-oligodendrocyte glycoprotein; My
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Devanjan Dey, Sagar Tyagi, Vadanya Shrivastava, Sweety Rani, Jai Bhagwan Sharma,Subrata Sinha,Jayanth Kumar Palanichamy,Pankaj Seth,Sudip Sen Using Human Fetal Neural Stem Cells to Elucidate the Role of the JAK-STAT Cell Signaling Pathway in Oligodendrocyte Differentiation In Vitro Mol Neurobiol, (2024)

PubMed: 38227271

Applications

WB

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (orb11068) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (orb11068) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Myelin-oligodendrocyte glycoprotein (orb11068) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 26 kD.

Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Myelin-oligodendrocyte glycoprotein (orb11068) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 26 kD.

Sample: Lane 1: Mouse Spinal cord tissue lysates, Lane 2: Rat Spinal cord tissue lysates, Primary: Anti-MOG (orb11068) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kDa, Observed band size: 26 kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MOG Polyclonal Antibody, Unconjugated (orb11068) 1:200, overnight at 4⒉C, followed by conjugation to the secondary antibody and DAB staining.