MNX1/HLXB9 Rabbit Polyclonal Antibody

SKU: orb157435

Description

MNX1/HLXB9 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500, Flow-Cyt=1ug/test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HLXB9 (231-330/401aa)
• Target: MNX1
• Molecular Weight: 41 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

HB9/HLXB9; HB 9; HB9; HLXB 9; HLXB9; Homeo box HB9; Homeobox HB9; Homeobox protein HB9; HOXHB9; MNX1; MNX1_HUMAN; Motor neuron and pancreas homeobox protein 1; Sacral agenesis autosomal dominant(Currarino triad); SCRA 1; SCRA1; SCRA1; HOXHB9.

Black line: Positive blank control RSC96), Negative blank control (HL60) Green line: Primary Antibody (Rabbit Anti-HLXB9 antibody (orb157435)) Orange line: Isotype Control Antibody (Rabbit IgG). Blue line: Secondary Antibody (Goat anti-rabbit IgG-AF488) RSC96 (Positive) and HL60 (Negative control) cells (black) were fixed with 4% PFA for 10 min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HLXB9 Antibody (orb157435) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Blank control: K562. Primary Antibody (green line): Rabbit Anti-MNX1 antibody (orb157435), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-MNX1 antibody (orb157435), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

K562 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MNX1) polyclonal Antibody, Unconjugated (orb157435) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Positive control: RSC96, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 6 µg in 100 µl 1X PBS containing 0.5% BSA.

Sample: Lane 1: Pancreas (Mouse) Lysate at 40 ug, Lane 2: Pancreas (Rat) Lysate at 40 ug, Primary: Anti-MNX1/HLXB9 (orb157435) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.

Quick Database Links

Gene Symbol

MNX1

UniProt

P50219

References

Chen, Lingling et al. Conducting molybdenum sulfide/graphene oxide/polyvinyl alcohol nanocomposite hydrogel for repairing spinal cord injury J Nanobiotechnology, 20, 210 (2022)

PubMed: 35524268