MMP2 Rabbit Polyclonal Antibody

SKU: orb11061

Description

MMP2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:200-800, IHC-F=1:200-800, ICC/IF=1:100-500, IF=1:200-800, Flow-Cyt=1μg/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MMP2 (31-109/476aa)
• Target: MMP2
• Molecular Weight: 72 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

MMP-2; 72 kDa gelatinase; 72kD type IV collagenase; CLG 4; CLG 4A; CLG4; CLG4A; Collagenase Type 4 alpha; Collagenase type IV A; Gelatinase A; Gelatinase alpha; Gelatinase neutrophil; Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase; Matrix metalloproteinase 2(gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); Matrix Metalloproteinase 2; Matrix metalloproteinase II; MMP 2; MMP II; MONA; Neutrophil gelatinase; TBE 1; MMP2_HUMAN.

Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-MMP2 antibody (orb11061), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 80% methanol (5 min at -20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-MMP2 antibody (orb11061), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MMP2) Polyclonal Antibody, Unconjugated (orb11061) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MMP2) Polyclonal Antibody, Unconjugated (orb11061) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Glioma, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated (orb11061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated (orb11061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: A431 (Human) Cell Lysate at 30 ug, Mcf-7 (Human) Cell Lysate at 30 ug, Primary: Anti-MMP-2 (orb11061) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Sample: SY5Y (Human) Cell Lysate at 30 ug, U2os (Human) Cell Lysate at 30 ug, U251 (Human) Cell Lysate at 30 ug, Siha (Human) Cell Lysate at 30 ug, Primary: Anti-MMP-2 (orb11061) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 60 kD.

Tissue/Cell: U-87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MMP2) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

MMP2

UniProt

P08253

References

Shenglan Wang 1, Chunhong Huang 1, Yanbin Zheng 1, Xinjie Wu 2, Yutong Zhong NPAS2, transcriptionally activated by ARRB1, promotes the malignant behaviours of lung adenocarcinoma cells and regulates the reprogramming of glucose metabolism Clin Exp Pharmacol Physiol ., (2024)

PubMed: 38584327

Applications

WB

Reactivity

Human

Jie Zhong 1 2, Yingzi Guo 1 2, Wang Xin EMMPRIN aggravates angiogenesis and blood-retina barrier injury by regulating matrix metalloproteinases in diabetic retinopathy Diab Vasc Dis Res, (2025)

PubMed: 40251743

Applications

WB

Reactivity

Rat