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MMP2 Rabbit Polyclonal Antibody

Catalog Number: orb11061

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11061
CategoryAntibodies
DescriptionMMP2 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Equine, Porcine, Sheep
ReactivityHuman, Mouse, Rabbit, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human MMP2 (31-109/476aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1μg/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW72 kDa
TargetMMP2
UniProt IDP08253
RRIDAB_10748736
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesMMP-2; 72 kDa gelatinase; 72kD type IV collagenase
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NoteFor research use only
Expiration Date12 months from date of receipt.

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Human
MMP2 Rabbit Polyclonal Antibody

Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-MMP2 antibody (orb11061), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 80% methanol (5 min at -20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

MMP2 Rabbit Polyclonal Antibody

Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-MMP2 antibody (orb11061), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test.

MMP2 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MMP2) Polyclonal Antibody, Unconjugated (orb11061) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

MMP2 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MMP2) Polyclonal Antibody, Unconjugated (orb11061) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

MMP2 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Glioma, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated (orb11061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

MMP2 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated (orb11061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

MMP2 Rabbit Polyclonal Antibody

Sample: A431 (Human) Cell Lysate at 30 ug, Mcf-7 (Human) Cell Lysate at 30 ug, Primary: Anti-MMP-2 (orb11061) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

MMP2 Rabbit Polyclonal Antibody

Sample: SY5Y (Human) Cell Lysate at 30 ug, U2os (Human) Cell Lysate at 30 ug, U251 (Human) Cell Lysate at 30 ug, Siha (Human) Cell Lysate at 30 ug, Primary: Anti-MMP-2 (orb11061) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 60 kD.

MMP2 Rabbit Polyclonal Antibody

Tissue/Cell: U-87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MMP2) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

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    ICC,  IHC-Fr,  IHC-P,  IP,  WB

    Human, Mouse, Porcine, Rat

    Rabbit

    Polyclonal

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  • MMP-2 Rabbit Polyclonal Antibody [orb101824]

    FC,  ICC,  IF,  IHC-Fr,  IHC-P,  WB

    Bovine, Equine, Porcine, Sheep

    Human, Mouse, Rabbit, Rat

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    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
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    FC,  ICC,  IF,  IHC-Fr,  IHC-P,  WB

    Bovine, Equine, Porcine, Sheep

    Human, Mouse, Rabbit, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
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    IF,  IHC-P,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

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    IHC,  WB

    Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl