MMP2 Rabbit Polyclonal Antibody

• Catalog Number: orb101049
• Category: Antibodies
• Description: MMP2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Porcine, Sheep
• Reactivity: Human, Mouse, Rabbit, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human MMP-2 PEX (561-660/660aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 24/62 kDa
• Target: MMP2
• UniProt ID: P08253
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PEX; MMP-2; MMP 2; 72 kDa gelatinase; 72kD type IV
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Xu, Min et al. Anti-CD47 monoclonal antibody therapy reduces ischemia-reperfusion injury of renal allografts in a porcine model of donation after cardiac death Am J Transplant, 18, 855-867 (2018)

PubMed: 29087049

Applications

IHC, WB

Reactivity

Porcine

Buile, Dace et al. Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients Pediatr Rep, 14, 419-433 (2022)

PubMed: 36278554

Applications

IHC

Reactivity

Human

Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-MMP2 antibody (orb101049), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/test.

Blank control: U-87MG (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-MMP2 antibody (orb101049), Dilution: 5 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Sample: HT1080 Cell (Human) Lysate at 40 ug, Primary: Anti-MMP2 (orb101049) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24/62 kD, Observed band size: 64 kD.

Tissue/cell: U-87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (MMP2) polyclonal Antibody, Unconjugated (orb101049) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.