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MMP13 Recombinant Protein (Human)
Catalog Number: orb2026201
| Catalog Number | orb2026201 |
|---|---|
| Category | Proteins |
| Description | MMP13 Recombinant Protein (Human) |
| Species/Host | E. coli |
| Form/Appearance | Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20 uM ZnCl2, 0.5% Brij-35, and 30% glycerol. |
| Purity | > 95% (SDS-PAGE) |
| MW | 20 kDa |
| UniProt ID | P45452 |
| Biological Activity | Preincubation of MMP-13 catalytic domain at 13nM with the broad-spectrum inhibitor GM6001 at 20nM for 1 hour completely inhibits enzymatic activity. Specific Activity: ≥100 U/µg. One U=100 pmol/min at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100 µM) as substrate. |
| NCBI | NP_003875.3 |
| Storage | Store at -8°C |
| Buffer/Preservatives | Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20 uM ZnCl2, 0.5% Brij-35, and 30% glycerol. |
| Alternative names | CLG3;collagenase 3;MANDP1;matrix metalloproteinase Read more... |
| Note | For research use only |
| Application notes | Application Info: Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors. |
| Expiration Date | 6 months from date of receipt. |
Human MMP13 protein [orb246393]
Greater than 90% as determined by SDS-PAGE.
58.3 kDa
E.coli
20 μg, 100 μg, 1 mgMouse MMP13 Protein, hFc Tag [orb1173668]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 78.1 kDa after removal of the signal peptide. The apparent molecular mass of mMMP13-hFc is approximately 70-100 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgHuman MMP13 Protein, hFc Tag [orb1173676]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 77.8 kDa after removal of the signal peptide. The apparent molecular mass of MMP13-hFc is approximately 70-100 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgHuman MMP13 Protein, His Tag [orb1173677]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 52.5 kDa after removal of the signal peptide. The apparent molecular mass of MMP13-His is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgMMP13 Recombinant Protein (Human) [orb2054052]
Greater than 90% as determined by SDS-PAGE.
58.3 kDa
E.coli
20 μg
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