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Catalog Number | orb1958711 |
---|---|
Category | Proteins |
Description | MMP-9 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 77.7 kDa and the accession number is P14780. |
Tag | C-His |
Purity | 97.70% |
UniProt ID | P14780 |
MW | 77.7 kDa (predicted); 80-95 kDa (reducing condition, due to glycosylation) |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | MMP-9 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 77.7 kDa and the accession number is P14780. |
Expression Region | A DNA sequence encoding the pro form of human MMP9 enzyme (NP_004985.2) (Met 1-Asp 707) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Ala 20 |
Storage | -20°C |
Note | For research use only |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 77.2 kDa after removal of the signal peptide. The apparent molecular mass of MMP9(20-707)-His is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
> 90% as determined by SDS-PAGE | |
95 kDa |
> 90% as determined by SDS-PAGE | |
24 kDa |
> 95% as determined by SDS-PAGE | |
32 kDa |
> 90% as determined by SDS-PAGE | |
36 kDa |