MMLV RT Protein

SKU: orb90183

Description

Recombinant Moloney Murine Leukemia Virus Reverse Trancscriptase protein

Images & Validation

• Application Notes:
  Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37oC, using poly(A)-oligo(dT)12-18 as the template-primer. Standard cDNA Synthesis Conditions50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2 mg radom hexamer,1-5mg RNA, 200units M-MLV RT. The reaction volume was 20ml and the incubation was 45 min at 42oC

Key Properties

• Source: Recombinant E. coli strain

Storage & Handling

• Storage: Stability: Stable for 5 days at 10°C, for longer period of time store at -20°C. Please prevent freeze-thaw cycles
• Form/Appearance: Sterile Filtered clear solution (200 U/µl).
• Buffer/Preservatives: 50mM Tris-HCl, 0.1M NaCl, 0.1% Triton X-100, 2mM DTT, 0.1mM EDTA and 50% glycerol.
• Disclaimer: For research use only