Mlph Antibody

• Catalog Number: orb1247271
• Category: Antibodies
• Description: Mlph Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, Pull-down Assay, WB
• Predicted Reactivity: Rat
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: C-ARHIFAKPVMAQQP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2ug/ml. Please note that this product may require overnight incubation of primary and high protein loads. This antibody has been successfully used in WB on Mouse: Sanzà Pet al (PMID: 30898842), Singh RK et al (PMID: 23281710), Figueiredo AC et al (PMID: 18559336) and Hume AN et al (PMID: 16914517).Immunofluorescence: Successfully used at 0.5ug/ml to stain mouse melanocytes. This antibody has been successfully used in IF on Mouse: Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. PMID:18559336. GST-pull down assay: See publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. (PMID:18559336).
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Mlph
• UniProt ID: Q91V27
• NCBI: NP_443748.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: 2210418F23Rik, melanophilin, Mlph, ln, l1Rk3, Slac
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2ug/ml. Please note that this product may require overnight incubation of primary and high protein loads. This antibody has been successfully used in WB on Mouse: Sanzà Pet al (PMID: 30898842), Singh RK et al (PMID: 23281710), Figueiredo AC et al (PMID: 18559336) and Hume AN et al (PMID: 16914517).Immunofluorescence: Successfully used at 0.5ug/ml to stain mouse melanocytes. This antibody has been successfully used in IF on Mouse: Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. PMID:18559336. GST-pull down assay: See publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. (PMID:18559336).
• Expiration Date: 12 months from date of receipt.

orb1247271 staining (0.5 ug/ml) of total melanocyte PNS (200 ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface.

Wild-type C57bl6 derived melanocytes were stained with orb1247271 (0.5 ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution.