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Product Overview
Product Name Mlkl antibody
Catalog Number orb32399
Species/HostRabbit
ReactivityGuinea pig, Human, Mouse, Rat
ConjugationUnconjugated
Custom conjugations
Tested applicationsICC, IF, IHC-P, WB
Immunogen

KLH conjugated synthetic peptide derived from human Mlkl. Please contact us for the exact immunogen sequence. The peptide is available as orb374793.

Target Mlkl
Alternative Names
Product Properties
Form/Appearance 10 mM PBS, 0.02% sodium azide
Concentration

- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml

Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity

Polyclonal antibodies are purified by peptide affinity chromatography

MW 54 kDa
Uniprot ID

Q8NB16 (Human); Q9D2Y4 (Mouse); D3ZKP6 (Rat)

NCBI NP_001135969.1; NM_001142497.2
Entrez 197259
Product Description

Rabbit polyclonal antibody to MlKl (mixed lineage kinase domain-like) is a 471 amino acid protein that contains one protein kinase domain which is thought to be catalytically inactive. Chromosome 16, which is associated with a variety of genetic disorders, encodes over 900 genes and comprises nearly 3% of the human genome. The rare disorder Rubinstein-Taybi syndrome is associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition.

Application Notes
Dilution Range WB: 1:200-2000, IHC-P: 1:100-500, IF/ICC: 1:100-500
Validation Images
IHC-P staining of guinea pig heart tissue using anti-Mlkl (2.5 ug/ml)
IHC-P staining of guinea pig heart tissue using anti-Mlkl (2.5 ug/ml)
Immunofluorescence image of guinea pig heart tissue using anti-Mlkl (2.5 ug/ml)
Immunofluorescence image of guinea pig heart tissue using anti-Mlkl (2.5 ug/ml)
Western blot analysis of human breast tumour (lane 1), human thyroid tumour (lane 2), human lung cancer (lane 3), Hela cells (lane 4) using Mlkl antibody (1 ug/ml)
Western blot analysis of human breast tumour (lane 1), human thyroid tumour (lane 2), human lung cancer (lane 3), Hela cells (lane 4) using Mlkl antibody (1 ug/ml)
Immunohistochemical staining of paraffin embedded guinea pig lung tissue using Mlkl antibody (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded guinea pig lung tissue using Mlkl antibody (2.5 ug/ml)
IF analysis of guinea pig heart tissue using anti-Mlkl (2.5 ug/ml)
IF analysis of guinea pig heart tissue using anti-Mlkl (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat stomach tissue using Mlkl antibody (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat stomach tissue using Mlkl antibody (2.5 ug/ml)
WB analysis of rat heart (lane 1), mouse stomach (lane 2), rat liver (lane 3), rat spleen (lane 4), rat lung (lane 5), human breast cancer (lane 6) using Mlkl antibody (1 ug/ml)
WB analysis of rat heart (lane 1), mouse stomach (lane 2), rat liver (lane 3), rat spleen (lane 4), rat lung (lane 5), human breast cancer (lane 6) using Mlkl antibody (1 ug/ml)
IHC-P staining of mouse lymph node tissue using anti-Mlkl (2.5 ug/ml)
IHC-P staining of mouse lymph node tissue using anti-Mlkl (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat thymus tissue using anti-Mlkl (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat thymus tissue using anti-Mlkl (2.5 ug/ml)
IHC-P image of human thyroid tumour tissue using Mlkl antibody (2.5 ug/ml)
IHC-P image of human thyroid tumour tissue using Mlkl antibody (2.5 ug/ml)
Immunohistochemical staining of rat skin tissue using Mlkl antibody (2.5 ug/ml)
Immunohistochemical staining of rat skin tissue using Mlkl antibody (2.5 ug/ml)
IHC-P staining of human breast tumour tissue using Mlkl antibody (2.5 ug/ml)
IHC-P staining of human breast tumour tissue using Mlkl antibody (2.5 ug/ml)
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