Mlkl antibody

SKU: orb32399

Description

Mlkl antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-P, WB
• Dilution range: WB: 1:200-2000, IHC-P: 1:100-500, IF/ICC: 1:100-500

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Mlkl. Please contact us for the exact immunogen sequence. The peptide is available as orb374793.
• Target: Mlkl
• Molecular Weight: 54 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti-Mixed lineage kinase domain-like protein antibody, anti-Mlkl antibody

IHC-P staining of human breast tumour tissue using Mlkl antibody (2.5 ug/ml)

Immunohistochemical staining of rat skin tissue using Mlkl antibody (2.5 ug/ml)

IHC-P image of human thyroid tumour tissue using Mlkl antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat thymus tissue using anti-Mlkl (2.5 ug/ml)

IHC-P staining of mouse lymph node tissue using anti-Mlkl (2.5 ug/ml)

WB analysis of rat heart (lane 1), mouse stomach (lane 2), rat liver (lane 3), rat spleen (lane 4), rat lung (lane 5), human breast cancer (lane 6) using Mlkl antibody (1 ug/ml)

Immunohistochemical staining of paraffin embedded rat stomach tissue using Mlkl antibody (2.5 ug/ml)

IF analysis of guinea pig heart tissue using anti-Mlkl (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded guinea pig lung tissue using Mlkl antibody (2.5 ug/ml)

Western blot analysis of human breast tumour (lane 1), human thyroid tumour (lane 2), human lung cancer (lane 3), Hela cells (lane 4) using Mlkl antibody (1 ug/ml)

Immunofluorescence image of guinea pig heart tissue using anti-Mlkl (2.5 ug/ml)

IHC-P staining of guinea pig heart tissue using anti-Mlkl (2.5 ug/ml)

Quick Database Links

Gene Symbol

Mlkl

UniProt

D3ZKP6, Q9D2Y4, Q8NB16

NCBI Gene ID

197259

RefSeq:001135969, 001142497

References

Giusy Rita Caponio 1, Alessandro Annunziato 2, Mirco Vacca 2, Graziana Difonzo 2, Giuseppe Celano 2, Fabio Minervini 2, Marianna Ranieri 1, Giovanna Valenti 1, Grazia Tamma 1, Maria De Angelis Nutritional, antioxidant and biological activity characterization of orange peel flour to produce nutraceutical gluten-free muffins Food Funct, (2024)

PubMed: 39052071

Xing Fan 1, Fang Lin 1, Yu Chen 2, Yuling Dou 1, Ting Li 1 3, Xinxin Jin 4, Jintao Song 2, Fang Wang Luteolin-7- O-β-d-glucuronide Ameliorates Cerebral Ischemic Injury: Involvement of RIP3/MLKL Signaling Pathway Molecules, (2024)

PubMed: 38611943

Applications

IF, WB

Reactivity

Rat

Sublethal necroptosis signaling promotes inflammation and liver cancer Immunity, S1074-7613(23)00234-0 (2023)

PubMed: 37329888

Zhang, Liansheng et al. BAD-mediated neuronal apoptosis and neuroinflammation contribute to Alzheimer's disease pathology iScience, 24, 102942 (2021)

PubMed: 34430820

Günther, Claudia et al. The pseudokinase MLKL mediates programmed hepatocellular necrosis independently of RIPK3 during hepatitis J Clin Invest, 126, 4346-4360 (2016)

PubMed: 27756058

Saleh, Danish et al. Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis Methods Mol. Biol., 1857, 19-33 (2018)

PubMed: 30136227

Zhou, Huiling et al. RIP1/RIP3/MLKL-mediated necroptosis contributes to vinblastine-induced myocardial damage Mol Cell Biochem, 476, 1233-1243 (2021)

PubMed: 33247805

Fuchslocher Chico, Johaiber et al. The enhanced susceptibility of ADAM-17 hypomorphic mice to DSS-induced colitis is not ameliorated by loss of RIPK3, revealing an unexpected function of ADAM-17 in necroptosis Oncotarget, 9, 12941-12958 (2018)

PubMed: 29560122

Bittel, Miriam et al. Modulation of the extrinsic cell death signaling pathway by viral Flip induces acute-death mediated liver failure Cell Death Dis, 10, 878 (2019)

PubMed: 31754092

Choi, Solji et al. 1-Palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol ameliorates chemoradiation-induced oral mucositis Oral Dis, 26, 111-121 (2020)

PubMed: 31677207

Frank, Tanja et al. Cell cycle arrest in mitosis promotes interferon-induced necroptosis Cell Death Differ, 26, 2046-2060 (2019)

PubMed: 30742091

Stolberg-Stolberg, Josef et al. Cartilage Trauma Induces Necroptotic Chondrocyte Death and Expulsion of Cellular Contents Int J Mol Sci, 21, 4204 (2020)

PubMed: 32545631