MLKL Antibody Discontinued

SKU: orb229539

Description

Rabbit polyclonal antibody to MLKL also known as hMLKL whose expression can be detected in the adult and fetal brain and kidney but is highly expressive in renal tubules and testis. It is found in the cytoplasm. This protein plays an efficient role in TNF-induced necroptosis, a programmed cell death process and is activated following phosphorylation by RIPK3 leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. It does not have protein kinase activity.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-9130019I15Rik antibody

Western blot analysis of THP-1 cell line using MLKL antibody

Guida, Natascia et al. The neurotoxicant PCB-95 by increasing the neuronal transcriptional repressor REST down-regulates caspase-8 and increases Ripk1, Ripk3 and MLKL expression determining necroptotic neuronal death Biochem Pharmacol, 142, 229-241 (2017)

PubMed: 28676433