MLF1 Antibody

SKU: orb1263697

Description

MLF1 Antibody

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Application Notes:
  For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This MLF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-244 amino acids from the C-terminal region of human MLF1.
• Target: MLF1
• Molecular Weight: 31 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1

Western blot analysis of MLF1 using rabbit polyclonal MLF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLF1 gene.

Western blot analysis in MCF-7 cell line lysates (35 ug/lane).

Western blot analysis of MLF1 Antibody in mouse heart tissue lysates (35 ug/lane)

Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

MLF1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

MLF1

UniProt

P58340

RefSeq:P58340