MKP1 Rabbit Polyclonal Antibody

SKU: orb13399

Description

MKP1 Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DUSP1 (151-250/367aa)
• Target: DUSP1
• Molecular Weight: 39 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CL100; HVH1; MKP-1; MKP1; PTPN10; 3CH134; Ptpn16; erp; DUS1_HUMAN; DUSP1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1 (MAP kinase phosphatase 1 | MKP-1); Protein-tyrosine phosphatase CL100; 3.1.3.16; 3.1.3.48; VH1; DUS1_MOUSE; Protein-tyrosine phosphatase 3CH134; Protein-tyrosine phosphatase ERP; DUS1_RAT; Protein-tyrosine phosphatase non-receptor type 16;

Paraformaldehyde-fixed, paraffin embedded (Mouse colon), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (MKP1) Polyclonal Antibody, Unconjugated (orb13399) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (MKP1) Polyclonal Antibody, Unconjugated (orb13399) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Pancreas (Mouse) Lysate at 30 ug, Primary: Anti-MKP1 (orb13399) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1:5000 dilution, Predicted band size: 39kD, Observed band size: 39kD.

Quick Database Links

Gene Symbol

DUSP1

UniProt

P28562