Mitofilin Rabbit Polyclonal Antibody

• Catalog Number: orb13508
• Category: Antibodies
• Description: Mitofilin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Mitofilin (241-340/758aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: IMMT
• UniProt ID: Q16891
• RRID: AB_10752720
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Cell proliferation inducing protein 52; Cell proli
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Human HepG2 cells fixed with 3.7% Formaldehyde in medium, blocking performed with 10% NGS in 1x PBS buffer + 0.1% Tween20. Incubated with Anti-Mitofilin/IMMT Polyclonal Antibody (orb13508) at a 1:10 dilution for 1hr at RT, followed by Anti-Rabbit secondary, Cy3 conjugate at a 1:300 dilution incubated at RT for 1hr.

Human HepG2 cells fixed with 3.7% Formaldehyde in medium, blocking performed with 10% NGS in 1x PBS buffer + 0.1% Tween20. Incubated with Anti-Mitofilin/IMMT Polyclonal Antibody (orb13508) at a 1:10 dilution for 1hr at RT, followed by Anti-Rabbit secondary, Cy3 conjugate at a 1:300 dilution incubated at RT for 1hr.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Mitofilin) Polyclonal Antibody, Unconjugated (orb13508) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (itofilin) Polyclonal Antibody, Unconjugated (orb13508) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Mitofilin) Polyclonal Antibody, Unconjugated (orb13508) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A673 (Human) Cell Lysate at 30 ug, Primary: Anti-Mitofilin (orb13508) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD.