Mitochondrial Marker Antibody

• Catalog Number: orb2318376
• Category: Antibodies
• Description: Mitochondrial Marker Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MTC754
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Mitochondrial fraction of HeLa cells
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (0.25-0.5ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 60kDa
• Entrez: Not Known
• Expression System: CHO cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Not Known
• Note: For research use only
• Application notes: Positive Control: HeLa or HepG2 cells. Hepatic carcinomas
• Expiration Date: 12 months from date of receipt.