MIF Antibody

• Catalog Number: orb234320
• Category: Antibodies
• Description: MIF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MIF recombinant protein (Position: P2-A115). Human MIF shares 89% and 90% amino acid (aa) sequence identity with mouse and rat MIF respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry , 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 12476 MW
• UniProt ID: P14174
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Macrophage migration inhibitory factor;MIF;5.3.2.1
• Note: For research use only
• Application notes: WB: The detection limit for MIF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of Jurkat cells using anti-MIF antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of MIF using anti-MIF antibody.Lane 1:human Jurkat cell;2:human K562 cell;3:human HeLa cell; 4:human HepG2 cell.

IF analysis of MIF using anti-MIF antibody. MIF was detected in an immunocytochemical section of HeLa cells.

IHC analysis of MIF using anti-MIF antibody. MIF was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of MIF using anti-MIF antibody. MIF was detected in a paraffin-embedded section of human lung cancer tissue.