Microphthalmia Transcription Factor antibody

• Catalog Number: orb108818
• Category: Antibodies
• Description: Purified monoclonal antibody clone C5 in phosphate buffered saline with 0.08% sodium azide
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C5
• Tested applications: IHC-Fr, IHC-P, IP, WB
• Reactivity: Human, Rat
• Isotype: IgG1
• Immunogen: N-terminal fragment of human microphthalmia protein.
• Concentration: See vial for concentration
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• UniProt ID: O75030
• Source: Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.
• Storage: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
• Alternative names: MiTF
• Note: For research use only
• Application notes: This antibody can be used for gel supershift assays, immunoprecipitation (2 µg/mg of protein lysate), Western blotting (1 µg/ml) and immunohistochemistry on frozen and formalin/paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions. In Western blotting, this antibody recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of tumor cells using Microphthalmia Transcription Factor antibody