MICB Rabbit Polyclonal Antibody

SKU: orb1241

Description

MICB Rabbit Polyclonal Antibody

Research Area

Epigenetics and Nuclear Signaling

Images & Validation

• Tested Applications: WB
• Dilution range: WB=1:500-2000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MICB (81-180/383aa)
• Target: MICB
• Molecular Weight: 40 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

MHC class I polypeptide related sequence B; MIC-B; MIC B; PERB11.2; MHC class I chain related protein B2; MHC class I mic B antigen; MICB_HUMAN.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human U266 cell lysates, Lane 3: Human Jurkat cell lysates, Lane 4: Human HUVEC cell lysates, Primary: Anti-MICB (orb1241) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kDa, Observed band size: 60 kDa.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MICB Polyclonal Antibody, Unconjugated (orb1241) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

MICB

UniProt

Q29980