MIA/CD-RAP Antibody

SKU: orb1248524

Description

MIA/CD-RAP Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 14kDa band observed in Human Glioma lysates (calculated MW of 14.5kDa according to NP_006524.1). Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Skeletal Muscle. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: PKLADRKLCADQE
• Target: MIA / CD-RAP
• Molecular Weight: Approx 14kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MIA, melanoma inhibitory activity, CD-RAP, melanoma-derived growth regulatory protein

orb1248524 (1 ug/ml) staining of Human Glioma lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248524 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248524 (5 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

MIA / CD-RAP

UniProt

Q16674

RefSeq (Protein):NP_006524.1