MHC Class II Rabbit Polyclonal Antibody

• Catalog Number: orb157874
• Category: Antibodies
• Description: MHC Class II Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen DM beta chain (111-200/263aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 27 kDa
• Target: HLA-DMB
• UniProt ID: P28068
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HLA-DMB; HLA class II histocompatibility antigen,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Protein: mouse lung lysates at 30 ug, Primary: Anti-MHC Class II (orb157874) at 1:200, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000, ECL excitated the fluorescence, Predicted band size: 27kD, Observed band size: 30kD.

Sample: Lane 1: Lung (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Lymph node (Mouse) Lysate at 40 ug, Lane 4: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-MHC Class II (orb157874) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29/50 kD, Observed band size: 50 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MHC Class II Polyclonal Antibody, Unconjugated (orb157874) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.