MGMT Antibody

• Catalog Number: orb1535997
• Category: Antibodies
• Description: MGMT Antibody
• Target: MGMT
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 3.06 mg/ml
• Buffer/Preservatives: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol. PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (XP_005252739.1). MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN
• Tested applications: IF, IHC, IHC-P, WB
• Dilution range: IF (1:50 - 1:200), IHC (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000)
• Application notes: Further information: The predicted MW is 21kDa, while the observed MW by Western blot was 22kDa.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MGMT
• Note: For research use only

Immunofluorescence analysis of U2OS cells using MGMT antibody. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of HepG2 cells, using MGMT antibody at 1:1000 dilution.