MGLUR3 Rabbit Polyclonal Antibody

• Catalog Number: orb157870
• Category: Antibodies
• Description: MGLUR3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Equine, Human, Rabbit, Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Metabotropic Glutamate Receptor 3 (365-460/879aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=5μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 96 kDa
• Target: GRM3
• UniProt ID: Q14832
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: G protein coupled receptor family C group 1 member
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Mouse Liver Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-MGLUR3/PE Conjugated antibody, Dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/PE (orange), used under the same conditions.

Sample: Cerebral cortex (Mouse) Lysate at 40 ug, Primary: Anti-MGLUR3 (orb157870) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 96 kD, Observed band size: 116 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-MGLUR3 (orb157870) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 96 kD, Observed band size: 116 kD.

Sample: Hippocampus (Mouse) Lysate at 40 ug, Primary: Anti-MGLUR3 (orb157870) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 96 kD, Observed band size: 116 kD.