MGAT5 Rabbit Polyclonal Antibody

SKU: orb100294

Description

MGAT5 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Gallus, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MGAT5 (201-300/741aa)
• Target: MGAT5
• Molecular Weight: 85 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; 6-N-acetylglucosaminyltransferase; alpha 1, 3(6) mannosylglycoprotein; alpha 1, 6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase; alpha mannoside beta 1, 6 N acetylglucosaminyltransferase; alpha mannoside beta 1, 6 N acetylglucosaminyltransferase; alpha-mannoside beta-1, 6-N-acetylglucosaminyltransferase; alpha-1; alpha-1, 3(6)-mannosylglycoprotein; alpha-1, 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase; alpha-mannoside beta-1; alpha-mannoside beta-1, 6-N-acetylglucosaminyltransferase; beta 1, 6 N acetyl glucosaminyltransferase; beta-1, 6-N-acetyl-glucosaminyltransferase; GGNT5; GlcNAc T V; GlcNAc-T V; GNT V; GNT VA; GNT-V; GNTV; GNTVA; Mannoside acetylglucosaminyltransferase 5; Mannosyl(alpha 1, 6) glycoprotein beta 1, 6 N acetyl glucosaminyltransferase; mannosyl(alpha-1, 6-)-glycoprotein beta-1, 6-N-acetyl-glucosaminyltransferase; Mgat5; MGT5A_HUMAN; N acetylglucosaminyl transferase V; N acetylglucosaminyltransferase V mannosyl(alpha 1, 6) glycoprotein; N-acetylglucosaminyl-transferase V; N-acetylglucosaminyltransferase V.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (MGAT5) Polyclonal Antibody, Unconjugated (orb100294) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (MGAT5) Polyclonal Antibody, Unconjugated (orb100294) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-MGAT5 (orb100294) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 85 kD, Observed band size: 85 kD.

Quick Database Links

Gene Symbol

MGAT5

UniProt

Q09328

References

Cai, Jundong et al. miR-124-3p Regulates FGF2-EGFR Pathway to Overcome Pemetrexed Resistance in Lung Adenocarcinoma Cells by Targeting MGAT5 Cancer Manag Res, 12, 11597-11609 (2020)

PubMed: 33223850

Applications

WB

Reactivity

Human