MFGE8 Antibody

• Catalog Number: orb1252493
• Category: Antibodies
• Description: MFGE8 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MFG-06
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Canine, Human
• Isotype: IgG1, kappa
• Immunogen: A human milk fat globule membrane preparation was used as the immunogen for the MFGE8 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the MFGE8 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MFGE8
• UniProt ID: Q08431
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Lactadherin, Breast epithelial antigen BA46, HMFG,
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the MFGE8 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MFGE8 antibody (MFG-06).

FFPE human pancreas tested with MFGE8 antibody (MFG-06).

FFPE human lung carcinoma tested with MFGE8 antibody (MFG-06).

FFPE human renal cell carcinoma tested with MFGE8 antibody (MFG-06).

Western blot testing of MCF7 cell lysate with MFGE8 antibody (clone MFG-06).

SDS-PAGE Analysis of Purified, BSA-Free MFGE8 Antibody (clone MFG-06). Confirmation of Integrity and Purity of the Antibody.