MFGE8 Antibody

• Catalog Number: orb1252491
• Category: Antibodies
• Description: MFGE8 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EDM45
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: A delipidated human milk fat globule membrane preparation was used as the immunogen for the Lactadherin antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Lactadherin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MFGE8
• UniProt ID: Q08431
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Lactadherin, Breast epithelial antigen BA46, HMFG,
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Lactadherin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Lactadherin antibody (EDM45).

FFPE human lung carcinoma tested with Lactadherin antibody (EDM45).

FFPE human renal cell carcinoma tested with Lactadherin antibody (EDM45).

FFPE human pancreas tested with Lactadherin antibody (EDM45).

Western blot testing of MCF7 cell lysate with Lactadherin antibody (clone EDM45).