Methylmalonyl Coenzyme A mutase Antibody

• Catalog Number: orb389426
• Category: Antibodies
• Description: Methylmalonyl Coenzyme A mutase Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MUT (33-65aa LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPE), different from the related mouse sequence by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 83134 MW
• UniProt ID: P22033
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Methylmalonyl-CoA mutase, mitochondrial;MCM;5.4.99
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-MUT antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of MUT using anti-MUT antibody.Lane 1:human K562 cell;2:huam CACO-2 cell;3:rat liver tissue;4:rat kidney tissue;5:rat heart tissue;6:mouse liver tissue;7:mouse kidney tissue;8:mouse heart tissue.

IF analysis of MUT using anti-MUT antibody. MUT was detected in immunocytochemical section of U20S cells.

IHC analysis of MUT using anti-MUT antibody. MUT was detected in paraffin-embedded section of human mammary cancer tissues.

IHC analysis of MUT using anti-MUT antibody. MUT was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of MUT using anti-MUT antibody. MUT was detected in paraffin-embedded section of human lung cancer tissues.