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Catalog Number | orb654292 |
---|---|
Category | Antibodies |
Description | Methylmalonyl Coenzyme A mutase Antibody (monoclonal, 2D6) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2D6 |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG2b |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human MUT (33-65aa LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPE), different from the related mouse sequence by one amino acid. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 83 kDa |
UniProt ID | P22033 |
Sensitivity | > 5000 cells |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | MCM; Mut Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (orb654292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HeLa whole cell lysates; Lane 2: human K562 whole cell lysates; Lane 3: human HEK293 whole cell lysates; Lane 4: human PC-3 whole cell lysates; Lane 5: human Caco-2 whole cell lysates; Lane 6: human Raji whole cell lysates; Lane 7: rat PC-12 whole cell lysates; Lane 8: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Methylmalonyl Coenzyme A antigen affinity purified monoclonal antibody (Catalog # orb654292) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for Methylmalonyl Coenzyme A at approximately 83KD. The expected band size for Methylmalonyl Coenzyme A is at 83KD.
IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (orb654292). Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (orb654292) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90443) with DAB as the chromogen.
IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (orb654292). Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (orb654292) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90443) with DAB as the chromogen.
IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (orb654292). Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (orb654292) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90443) with DAB as the chromogen.
IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (orb654292). Methylmalonyl Coenzyme A was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (orb654292) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90443) with DAB as the chromogen.
IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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