MET/C-MET/HGFR Antibody

• Catalog Number: orb1294310
• Category: Antibodies
• Description: Rabbit polyclonal antibody to c-MET
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:500–1:1000 Immunofluorescence 1:100–1:200 Immunohistochemistry (Paraffin) 1:100
• Conjugation: Unconjugated
• Target: MET / C-MET / HGFR
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti c-MET antibody at 1/700 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4°C.

All lanes: Anti c-MET antibody at 1/700 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4°C.

All lanes: Anti c-MET antibody at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4°C.

All lanes: Anti c-MET antibody at 1/800 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4°C.

All lanes: Anti c-MET antibody at 1/800 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling c-MET with orb1294310 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling c-MET with orb1294310 at 1/100.