Mer2 Antibody

SKU: orb345543

Description

Mer2 antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution range: ELISA: 1:5,000 - 1:25,000, IP: 1:100, WB: 1:1,000 - 1:10,000
• Reactivity: Yeast
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to Mer2 protein by western blotting in the appropriate cell lysate or extract. This antibody is reactive with both phosphorylated and unphosphorylated Mer2 at the S30 position.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.
• Purity: This affinity-purified antibody is directed against the Saccharomyces cerevisiae Mer2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against Saccharomyces cerevisiae Mer2 protein and reactivity is independent of phosphorylation at residue S30. A BLAST analysis was used to suggest minimal cross reactivity with Mer2 homologues from other sources.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.75 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Mer2 antibody, Meiotic recombination 2 protein, Recombination protein 107, REC107, YJR021C, J1462

Western blot using Biorbyt's affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphorylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5000 dilution.

Quick Database Links

UniProt

P21651

RefSeq:1170924