Melan A Specific antibody

SKU: orb2276541

Description

Melan A antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Skin: Virtually all melanocytes should show a strong Melan A- immunostaining including a weak to moderate staining in melanocytic dendrites. Negative Control: Kidney: Melan A staining must be absent in all cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: MSVA-900M
• Immunogen: Recombinant full-length human MART-1 protein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA

Melan A staining is absent in the kidney.

Skin: In the skin, a strong Melan-A immunostaining is seen in melanocytes.

Skin Malignant melanoma with strong Melan A positivity in all tumor cells.

Quick Database Links

UniProt

Q16655